UK GCSE level age ~14-16, ~US grades 9-10 Biology revision notes
Enzymes: 6. The principles and methods of measuring the rate of enzyme catalysed reactions
Doc Brown's Biology exam study revision notes
There are various sections to work through, after 1 they can be read and studied in any order.
6. Methods of measuring enzyme activity
This section is about ideas (e.g. 'fair test') and possible experimental procedures for investigating the factors that control the rates of enzyme catalysed chemical reactions.
I do not give detailed recipes for the experiments - just lots of ideas, there is much 'stuff' to think about!
See also 'decay' investigation using milk and lipase gcse biology revision notes more specific links
Its part of the web page Carbon cycle, nitrogen cycle, water cycle, decomposition - decay investigation
How might the factors controlling enzyme activity be investigated in the laboratory?
In principle you can measure the rate of an enzyme reaction in two ways:
The experiments described below can be adapted to look at the four main factors (variables) which can affect and control the speed of an enzyme catalysed reaction.
Theoretically there are four main variables you can investigate for any enzyme reaction.
All of the following can all affect the speed of an enzyme catalysed reaction.
This means three of the variables involved in an experimental investigation must be kept constant except for the variable you are investigating e.g.
Two methods for following an enzyme catalysed reaction are illustrated below, full details on other pages in Parts 7. and 8.
Above and below: Part 7. Method 1. involving collecting a gas given off for all the details.
Above and below: Part 8. Method 2 Measuring a colour change for all the details.
See also GCSE chemistry notes: How we measure the speed or rate of a chemical reaction
Summary of learning objectives and key words or phrases
Understand and describe the principles and methods of measuring the rate of an enzyme catalysed reaction, including fair test criteria and how to control variables.
(Enzymes are also dealt with in my GCSE chemistry notes chemistry - biotechnology)
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