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School Biology revision notes: Cloning 2. Embryo stem cells

GCSE level biology revision notes: Cloning 2. Making animal clones using embryo transplants and cloned stem cells

[Author © Dr WP Brown PhD: Doc Brown's biology exam revision notes suitable for students of UK IGCSE & GCSE level biology courses & ~ US grades 9-10 biology [page updated Feb 25th 2026 *]

[Key points and learning objectives for this page, after the main body of notes]

Sub-index of biology notes on cloning

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(2) Making animal clones using embryo transplants and stem cells

Farming uses many new methods thanks to advances in 'biotechnology' e.g.

Farmers can produce cloned offspring from their prize bulls and cows using embryonic transplants.

Sperm cells are removed from a prize bull and egg cells taken from a prize cow.

The sperm are used to artificially inseminate (artificially fertilise) the egg cells.

The embryo that develops can be split many times to form clones - but before any stem cells become specialised.

The cloned embryos can be then implanted into cows.

The embryos can then grow and develop in the normal natural way to produce lots of genetically identical baby calves.

'Theoretically', hundreds of ideal offspring can be derived every year from just one best bull and cow parents.


Key points and extended summary of important ideas

Based on the syllabus-specifications for students taking the AQA, Edexcel and OCR GCSE level biology examinations (~US grades 9-10).

Animal Cloning: Embryo Transplants and Stem Cells

Cloning in animals can be achieved using embryo transplants and stem cells, each with distinct procedures and applications.


1. Embryo Transplants

Embryo transplant cloning is commonly used in livestock breeding to produce genetically identical animals with desirable traits, such as high milk yield or disease resistance.

Procedure:

  1. Selection of Parent Animals – A desirable male and female are selected for breeding.

  2. Fertilization – The sperm and egg cells fuse to create an embryo.

  3. Early Embryo Development – The embryo is allowed to divide several times but is stopped before cells begin differentiating.

  4. Embryo Splitting – The developing embryo is split into multiple genetically identical embryos.

  5. Transplantation – These identical embryos are implanted into multiple surrogate mothers.

  6. Birth of Cloned Offspring – The surrogate mothers give birth to genetically identical offspring.

Uses of Embryo Transplants:

  • Livestock improvement: Ensuring consistent quality in farm animals.

  • Conservation: Increasing populations of endangered species.

  • Scientific research: Studying genetic influences on disease.


2. Cloning Using Stem Cells

Stem cell technology allows scientists to produce clones from adult cells rather than embryos, through techniques such as somatic cell nuclear transfer (SCNT).

Procedure (SCNT):

  1. Cell Collection – A somatic (body) cell is taken from the animal to be cloned.

  2. Enucleation – The nucleus of an unfertilized egg is removed, leaving an empty egg cell.

  3. Nuclear Transfer – The nucleus from the somatic cell is inserted into the empty egg.

  4. Cell Stimulation – An electric shock or chemical treatment stimulates the egg to start dividing.

  5. Embryo Development – The resulting embryo is implanted into a surrogate mother.

  6. Birth of the Clone – The offspring is genetically identical to the donor animal.

Uses of Stem Cell Cloning:

  • Preserving rare breeds or endangered species.

  • Medical research, such as cloning animals for drug testing.

  • Regenerative medicine, potentially producing patient-specific cells for treatment.


Both cloning methods have advantages, but ethical concerns exist around genetic diversity reduction, animal welfare, and potential risks in cloned populations.


Summary of learning objectives and key words or phrases

Know the procedure for making animal clones and be able to describe the use of embryo transplants and the medical uses of cloned stem cells.


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